P02 Generation of viral vectors for visualizing and manipulating synaptic micro-circuits
The virus platform will generate and purify recombinant adeno-associated viruses (rAAVs) and lentiviruses (LVs) for in vivo gene transfer defined by the specific scientific requirements of the consortium. Furthermore, the platform will develop rAAV-, LV- and recombinant Rabies virus-based techniques to characterize and manipulate synaptic microcircuits. Specifically we will develop novel, genetically modified Rabies virus variants allowing a light-induced activity modulation of defined monosynaptic circuits. This new technology, in combination with established physiological methods offers the unique possibility to visualize synaptically connected neuronal ensembles while monitoring and manipulating their activity (functional neuroconnectomics).